Drug Discov Ther. 2021;15(2):78-86. (DOI: 10.5582/ddt.2021.01036)

Factors related to the composition and diversity of wound microbiota investigated using culture-independent molecular methods: a scoping review

Kunimitsu M, Kataoka Y, Nakagami G, Weller C, Sanada H


All open wounds are often colonized by commensal microbes as a loss of skin can provide a ready portal of entry for microorganisms. Although the wound microbiota is known to be associated with wound infection and with delayed healing, the factors related to the formations of wound microbiota contributing to such poor clinical outcomes are not clear and have not led to effective infection prevention interventions. This review aimed to scope the factors related to the composition and diversity of wound microbiota that have been investigated using culture-independent molecular methods. Original articles on wound microbiota published from January 1986 to February 2020 were included in this review. Thirty-one articles met the inclusion criteria and were grouped according to wound types: chronic, acute, and animal model wounds. The factors identified were categorized according to patient characteristics, wound characteristics, treatment, and sampling. Although some studies reported the effect size of the factors, the values were small. No studies elucidated the mechanism of wound microbiota formation. The results of this scoping review highlight that the factors associated with the diversity of wound microbiota are poorly understood and that further studies are needed.

KEYWORDS: microbiota, biodiversity, sequence analysis, whole genome sequencing, wound infection

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