Drug Discov Ther. 2012;6(6):315-320. (DOI: 10.5582/ddt.2012.v6.6.315)

The synergistic effect of SaOS-2 cell extract and other bone-inducing agents on human bone cell cultivation.

Saif A, Wende K, Lindequist U


Human osteosarcoma cell line SaOS-2 is an osteoblastic cell model that contains factors like bone morphogenetic proteins necessary for initiating bone formation. The cell line also expresses high levels of osteoinductive activity. In contrast to highly complicated and expensive ways to identify, purify, and separate specific bone-inducing agents from SaOS-2 cells, lysate can be used as an alternative to isolated bone-stimulating factors. Lysates of SaOS-2 stimulate the activity of the alkaline phosphatase of human osteoblastic cells HOS 58 in vitro. In other words, they probably possess osteoinductive activity. Different serial concentrations of substances like dexamethasone and insulin were tested with and without a lysate of SaOS-2 cells to assay their synergistic action. Results showed that a lysate of the SaOS-2 cell line acts as a synergistic agent and increases the osteoinductive activity of known boneinducing agents. SaOS-2 cell lysate could be used in the future as a clinical agent to promote bone repair and possibly enhance osteointegration. Using SaOS-2 total cellular extract offers the possibility of lowering the effective dose of other bone-inducing agents.

KEYWORDS: Osteoinduction, bone alkaline phosphatase, SaOS-2 cell lysate, osteointegration, human osteosarcoma cells HOS 58, bone morphogenetic proteins, tissue-engineered bone

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