Drug Discov Ther. 2014;8(5):218-224. (DOI: 10.5582/ddt.2014.01033)
R-eriodictyol and S-eriodictyol exhibited comparable effect against H2O2-induced oxidative stress in EA.hy926 cells.
Li HZ, Li C, Shen T, Zhao LJ, Ren DM
Eriodictyol is a flavanone well-known for its antioxidative activity. Due to a chiral carbon atom in position C-2, eriodictyol always exist in racemic form. In order to study the antioxidant activity under H2O2-induced oxidative stress of each enantiomer, enantiomers of eriodictyol were resolved by high-performance liquid chromatography (HPLC), using a Chiral Amylose-C column as chiral stationary phase. Online coupling HPLC-circular dichroism (CD) method was used for the determination of elution order and the absolute configurations of the two eluates. The protective effects of racemic and enantiomeric eriodictyol against H2O2-induced cytotoxicity with EA.hy926 cells were tested. The results showed that the two enantiomers of eriodictyol and the corresponding racemate were equipotent, suggesting that the configuration of the C-2 chiral center does not influence the cytoprotective activity against H2O2-induced oxidative stress in EA.hy926 cells.